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  • Nematicidal Activity of the Volatilome of Eruca sativa on Meloidogyne incognita.

Nematicidal Activity of the Volatilome of Eruca sativa on Meloidogyne incognita.

Journal of agricultural and food chemistry (2015-06-18)
Nadhem Aissani, Pietro Paolo Urgeghe, Chrisostomos Oplos, Marco Saba, Graziella Tocco, Giacomo Luigi Petretto, Kodjo Eloh, Urania Menkissoglu-Spiroudi, Nikoletta Ntalli, Pierluigi Caboni
ABSTRACT

Research on new pesticides based on plant extracts, aimed at the development of nontoxic formulates, has recently gained increased interest. This study investigated the use of the volatilome of rucola (Eruca sativa) as a powerful natural nematicidal agent against the root-knot nematode, Meloidogyne incognita. Analysis of the composition of the volatilome, using GC-MS-SPME, showed that the compound (Z)-3-hexenyl acetate was the most abundant, followed by (Z)-3-hexen-1-ol and erucin, with relative percentages of 22.7 ± 1.6, 15.9 ± 2.3, and 8.6 ± 1.3, respectively. Testing of the nematicidal activity of rucola volatile compounds revealed that erucin, pentyl isothiocyanate, hexyl isothiocyanate, (E)-2-hexenal, 2-ethylfuran, and methyl thiocyanate were the most active with EC50 values of 3.2 ± 1.7, 11.1 ± 5.0, 11.3 ± 2.6, 15.0 ± 3.3, 16.0 ± 5.0, and 18.1 ± 0.6 mg/L, respectively, after 24 h of incubation. Moreover, the nematicidal activity of fresh rucola used as soil amendant in a containerized culture of tomato decreased the nematode infection in a dose-response manner (EC50 = 20.03 mg/g) and plant growth was improved. On the basis of these results, E. sativa can be considered as a promising companion plant in intercropping strategies for tomato growers to control root-knot nematodes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Isobutyl isothiocyanate, AldrichCPR
Sigma-Aldrich
cis-3-Hexene, ≥95.0%
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Butyl isothiocyanate, 99%
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2-Furoic acid, 98%
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DL-Sulforaphane, ≥90% (HPLC), synthetic, liquid
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Carbon disulfide, anhydrous, ≥99%
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Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
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Dimethyl sulfoxide, anhydrous, ≥99.9%
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2-Ethylfuran, ≥99%
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trans-2-Hexen-1-al, ≥95%, FCC, FG
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Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
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Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
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Dimethyl sulfoxide, for molecular biology
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Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
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Dimethyl sulfoxide, PCR Reagent
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Dimethyl sulfoxide, Vetec, reagent grade, 99%
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Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
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2-Ethylfuran, 97%
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trans-2-Hexen-1-al, 98%