SAB4200541

Anti-ADAR1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-136 kDa double-stranded RNA-binding protein, Anti-DRADA, Anti-DSH, Anti-G1P1, Anti-IFI-4, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Anti-adenosine deaminase acting on RNA 1-A, Anti-adenosine deaminase, RNA-specific, Anti-double-stranded RNA-specific adenosine deaminase, Anti-interferon-induced protein 4
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~110 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 10-20 μg using lysates of human HepG2 or HEK-293T cells.; indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells.; western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells.
• UniProt accession no.: P55265
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... ADAR(505134) ; human ... ADAR(103) ; mouse ... Adar(56417) ; rat ... Adar(81635)

Description

General description

Adenosine deaminase that acts in RNA (ADAR) exists in two isoforms namely, ADAR1 and ADAR2, in mammals. Both the isoforms are expressed in variety of tissues but in general, the pre-mRNA being edited represents receptors of the central nervous systems. ADAR1 has several isoforms which include the full length ADAR1 (150 kDa) and two functionally active short isoforms (80 and 110 kDa).

Immunogen

synthetic peptide corresponding to an internal s of human ADAR1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, pig and monkey.

Application

Anti-ADAR1 antibody produced in rabbit has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Adenosine deaminase that acts in RNA 1 (ADAR1) play an important role in A-to-I RNA editing. In addition, it is also implicated in differentiating cells of embryo and adult tissues, facilitating cell survival and permitting their further differentiation and maturation. Its role in embryonic development is specifically observed within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). ADAR1 plays a key role in hematopoietic stem cell maintenance. It also block interferon signaling, that might help organisms to fight against the harmful effects of interferon activation linked with various diseases, such as chronic inflammation, autoimmune disorders and cancer.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable