SAE0109
SAvPhire Monomeric Streptavidin alkaline phosphatase conjugate
Synonym(s):
SAVphire-AP, mSA-AP
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.47
Recommended Products
biological source
Escherichia coli
conjugate
alkaline phosphatase
clone
monoclonal
mol wt
15.5 kDa
technique(s)
immunohistochemistry: suitable (Paraffin)
General description
for Savphire monomeric streptavidin with an N-terminal His-tag and a C-terminal FLAG® tag.
Specificity
SAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with alkaline phosphatase is suitable for immunodetection applications. SAvPhire Monomeric Streptavidin is expressed with an N-terminal His-tag and a C-terminal FLAG® tag.
Species Reactivity Note: Predicted to react with all species.
Species Reactivity Note: Predicted to react with all species.
Immunogen
N/A
Application
SAvPhire Monomeric Streptavidin,Cat.#SAE0109, is a high-affinity recombinant variant of SA that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules and offers easier access to the desired targets, tested for use in IHC (Paraffin).
Quality
Evaluated by Immunohistochemistry (Paraffin) with Anti-NeuN antibody (ABN78) for detection of NeuN in mouse brain tissue sections.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:500 dilution Anti-NeuN (ABN78) was used to stain sections of mouse cerebral cortex and signal was detected with Savphire monomeric streptavidin alkaline phosphatase conjugate (1:500 dilution) and Vulcan Fast Red.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:500 dilution Anti-NeuN (ABN78) was used to stain sections of mouse cerebral cortex and signal was detected with Savphire monomeric streptavidin alkaline phosphatase conjugate (1:500 dilution) and Vulcan Fast Red.
Physical form
Monomeric Streptavidin in buffer containing 0.05 M Tris, 0.15 M NaCl, 1 mM MgCl2, 0.2% BSA, pH 8.0 with 0.1% sodium azide with 10% glycerol.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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