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Key Documents

07-1401-AF647

Sigma-Aldrich

Anti-GLUT-1 Antibody, CT, Alexa Fluor 647 Conjugate

from rabbit, ALEXA FLUOR 647

Synonym(s):

Solute carrier family 2, facilitated glucose transporter member 1, Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter, GLUT-1, CT, Alexa Fluor 488 Conjugate

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), monkey (based on 100% sequence homology), feline (based on 100% sequence homology), equine (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC2A1(6513)

General description

Solute carrier family 2, facilitated glucose transporter member 1 (UniProt P11166; also known as Glucose transporter type 1 erythrocyte/brain, GLUT-1, HepG2 glucose transporter, Human T-cell leukemia virus I and II receptor, Receptor for HTLV-1 and HTLV-2) is encoded by the SLC2A1 (also known as DYT9, DYT17, DYT18, EIG12, GLUT, GLUT1, HTLVR, GLUT1DS, PED) gene (Gene ID 6513) in human. Glucose transporters (GLUT-1 to GLUT-7) constitute a family of integral membrane glycoproteins that facilitate cellular glucose uptake. In addition to mediateing glucose uptake in adult tissues, GLUT-1 is the predominant glucose transporter in embryonic and fetal tissues. Two forms of GLUT-1 are found in human frontal cortex, the 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.

Specificity

This antibody recognizes GLUT-1.

Immunogen

Epitope: C-terminus
Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV).

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GLUT-1 in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-1401) is shown to be suitable also for ELISA, Western blotting, immunocytochemistry, and immunohistochemistry applications.
Research Category
Signaling
Research Sub Category
Developmental Signaling
This Anti-GLUT-1 Antibody, CT, Alexa Fluor 647 Conjugate is validated for use in Immunocytochemistry for the detection of GLUT-1.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GLUT-1 in A431 cells.

Target description

~54 kDa observed

Physical form

Affinity purified
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


Certificates of Analysis (COA)

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