Skip to Content
Merck
All Photos(1)

Key Documents

810211P

Avanti

C12-NBD Ceramide

Avanti Research™ - A Croda Brand 810211P, powder

Synonym(s):

N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-D-erythro-sphingosine

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C36H61N5O6
CAS Number:
202850-01-9
Molecular Weight:
659.90
UNSPSC Code:
12352211
NACRES:
NA.25

Assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810211P-1mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand 810211P

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C36H61N5O6/c1-2-3-4-5-6-7-8-9-10-12-15-18-21-24-33(43)31(29-42)38-34(44)25-22-19-16-13-11-14-17-20-23-28-37-30-26-27-32(41(45)46)36-35(30)39-47-40-36/h21,24,26-27,31,33,37,42-43H,2-20,22-23,25,28-29H2,1H3,(H,38,44)/b24-21+/t31-,33+/m0/s1

InChI key

SNOJCYCOPNIGIK-ULETYAGTSA-N

General description

C12 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD) ceramide is a long-chain fluorescent phospholipid. It is a natural analog of ceramide with C12 acyl chain length.

Application

C12-NBD Ceramide or N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-D-erythro-sphingosine has been used as a substrate in ceramidase activity assay. It is also suitable for the lipid binding assays and in cell culture experiments for endothelial cells to determine the pro-apoptotic intracellular ceramide generation.

Biochem/physiol Actions

C12-7-nitro-2-1,3-benzoxadiazol-4-yl (NBD) Ceramide is useful for measuring the alkaline and neutral ceramidase activity. It is involved in maintainingthe physical properties of ceramide required for cellular studies.

Packaging

5 mL Amber Glass Screw Cap Vial (810211P-1mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Apoptotic Sphingolipid Signaling by Ceramides in Lung Endothelial Cells
Medler TR, et al.
American Journal of Respiratory Cell and Molecular Biology, 38(6), 639-646 (2008)
VPS13A and VPS13C are lipid transport proteins differentially localized at ER contact sites
Nikit K, et al.
The Journal of Cell Biology, 217(10), 3625-3639 (2018)
M Tani et al.
Journal of biochemistry, 125(4), 746-749 (1999-04-02)
A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of
Effect of ceramide N-acyl chain and polar headgroup structure on the properties of ordered lipid domains (lipid rafts)
Meghan PS, et al.
Biochimica et Biophysica Acta, 1768(9), 2205-2212 (2007)
Megha et al.
Biochimica et biophysica acta, 1768(9), 2205-2212 (2007-06-19)
Ceramides are sphingolipids that greatly stabilize ordered membrane domains (lipid rafts), and displace cholesterol from them. Ceramide-rich rafts have been implicated in diverse biological processes. Because ceramide analogues have been useful for probing the biological function of ceramide, and may
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service