Superspher^® HPLC Columns

Section Overview:

• Introduction
• Surface Modifications for Superspher^® HPLC columns
       - Polar Columns
       - Non-polar Bonding
• Specifications of Superspher^® HPLC Columns

Introduction

Superspher^® HPLC columns are designed to provide highly efficient separations. Both theoretical calculations and practical experience indicate that these traditional fully porous high-performance spherical silica carriers (Type A silica), characterized by a mean particle size of 4 μm, deliver  a good balance of pressure to separation performance ratio. The theoretical plate count for Superspher^® HPLC columns exceeds 100,000 N/m. This makes them suitable for sample mixtures requiring high peak capacity.

The Superspher^® HPLC column packing material is offered in LiChroCART^® cartridges as well as Hibar^® RT columns. Both column hardwares are available in various lengths and internal diameters of 4 mm, 3 mm, and 2 mm. LiChroCART^® cartridges with inner diameters of 4.6 mm, 4 mm, 3 mm, and 2 mm are suitable for use with the re-usable manu-CART^® “4” cartridge holder. This allows the quick exchange of the column while the holder stays connected to the HPLC instrument as well as the direct integration of a guard cartridge.

Surface Modifications for Superspher^® HPLC columns

The Superspher^® packing material is available with various surface modifications, such as non-polar derivatives (RP-8, RP-8 endcapped, RP-18, RP-18 endcapped and RP-select B) and polar surface (Si 60).

Polar columns

• Superspher^® Si 60: A versatile HPLC fully porous spherical silica sorbent (Type A) with a pore size of 60 Å suited to applications in normal phase chromatography and optional for hydrophilic interaction chromatography (HILIC).

Non-Polar Bonding

• Superspher^® 60 RP-8: A reliable and versatile fully porous spherical silica gel support (Type A) with a pore size of 60 Å, produced using traditional methods. It possesses less hydrophobic C-8 reversed phase characteristics, making it suitable for the separation of neutral, acidic, and weakly basic compounds.
• Superspher^® 60 RP-8 endcapped: A dependable and versatile, traditionally manufactured spherical silica gel support with a pore size of 60 Å featuring less hydrophobic C-8 endcapped reversed phase characteristics, ideal for the separation of Amino Acids, Esters, and other neutral, acidic, and weakly basic compounds with too high retention on C18.
• Superspher^® 100 RP-18: A dependable and adaptable fully porous spherical silica gel support, (Type A) with a pore size of 100 Å and C-18 reversed phase characteristics, ideal for the separation of neutral, acidic, and weakly basic compounds.
• Superspher^® 100 RP-18 endcapped: A fully porous spherical silica gel support, (Type A) with a pore size of 100 Å with C-18 endcapped reversed phase properties, making it suitable for the separation of a very broad range of compounds including Aflatoxines, Aldehydes, Alkaloides, Antibiotics, Catecholamines, Esters, Lipides, Fatty acids, Flavonoides, Ketones, Nitrosamines, Nucleosides Nucleotides, PCB, small Peptides, hydrophobilc Pesticides, Phenols, Phthalates, Preservatives, Steroides, Sulfonamides, Sweeteners and Vitamins.
• Superspher^® 60 RP-select B: A highly reproducible, fully porous spherical silica gel support, (Type A) with a pore size of 100 Å characterized by reversed phase properties and the hydrophobicity associated with a C-8 phase. This carrier is optimized for the separation of basic compounds but is also suitable for the determination of neutral and acidic compounds.

Specifications of Superspher^® HPLC Columns

Packing material	USP Designation	Characteristics	Pore size	Pore volume (mL/g)	Spec. Surface area SBET (m2/g)	Carbon load (%)	Surface coverage (μmol/m2)	pH Stability	Max Temperature	Endcapped
Superspher® Si 60	L3	Spherical particles of full porous silica (Type A) with 4 µm particle size	60 Å	0.85	700	N.A	--	2 - 7.5	65°C	N.A
Superspher® 60 RP-8	L7	Spherical particles of full porous silica (Type A) with 4 µm particle size and octyl silane bonding	60 Å	1.25	350	12.5	4.04	2 - 7.5	65°C	No
Superspher® 60 RP-8 endcapped	L7	Spherical particles of full porous silica (Type A) with 4 µm particle size, octyl silane bonding and endcapped	60 Å	1.25	350	13.0	4.44	2 - 7.5	65°C	Yes
Superspher® 100 RP-18	L1	Spherical particles of full porous silica (Type A) with 4 µm particle size and Octadecylsilane bonding	100 Å	1.25	350	21.0	3.61	2 - 7.5	65°C	No
Superspher® 100 RP-18 endcapped	L1	Spherical particles of full porous silica (Type A) with 4 µm particle size and Octadecylsilane bonding endcapped	100 Å	1.25	350	21.6	4.09	2 - 7.5	65°C	Yes
Superspher® 60 RP-select B	L7	Spherical particles of full porous silica (Type A) with 4 µm particle size and octyl silane bonding optimized for the RP-separation of basic compounds	60 Å	0.9	360	11.5	3.55	2 - 7.5	65°C	No

Ordering Information

Column dimension
Length (mm)		ID (mm)	RP-18	RP-18 endcapped	RP-8	RP-8 endcapped	RP-select B	Si
LiChroCART® HPLC Cartridge [1 unit; * 3 units]
25	x	4	1.16039.0001*	1.16869.0001*
30	x	3					1.50233.0001
75	x	4	1.50980.0001*				1.50974.0001*
125	x	2		1.50198.0001			1.50197.0001
125	x	3	1.50792.0001	1.51909.0001			1.50791.0001
125	x	4	1.16051.0001	1.16855.0001	1.16052.0001	1.16854.0001	1.50975.0001	1.16054.0001
150	x	4.6					1.51432.7141
250	x	2		1.50193.0001			1.51308.0001
250	x	3	1.51299.0001	1.51910.0001			1.51288.0001
250	x	4	1.16056.0001	1.16858.0001	1.16010.0001	1.16857.0001	1.50973.0001	1.16009.0001
250	x	4.6					1.51431.7141
Validation Kits [3 LiChroCART® HPLC cartridges from 3 different sorbent batches]
125	x	3	1.50792.1003
250	x	4	1.16056.1003
Superspher®- Bulk Sorbents
10 g Sorbent in a glass bottle			1.19613.0010		1.19612.0010		1.19643.0010