LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5
CONDITIONS
column
Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)
mobile phase
[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile; (15:85, A:B)
flow rate
0.3 mL/min
pressure
1189 psi (82 bar)
column temp.
35 °C
detector
MS ESI(+), 100-1000 m/z
injection
1 μL
sample
300 ng/mL each in acetonitrile
Descripción
Analysis Note
HILIC mode on Ascentis Express OH5 provided rapid separation of neurotransmitters in highly MS-compatible mobile phases. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany