LC/MS Analysis of Melamine and Metformin on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 5 cm x 3 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 10 mM ammonium formate in water; [B] acetonitrile; (5:95, A:B)

flow rate

1.0 mL/min

pressure

551 psi (38 bar)

column temp.

25 °C

detector

ESI(+), SIR, m/z 127, 130

injection

2 μL

sample

1 mg/L