HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide
Materiales
Application
Referencia del producto
Descripción
Precios
standard
Referencia del producto
Descripción
Precios
D-Pantothenic acid hemicalcium salt
meets USP testing specifications, monograph mol wt. 476.53 (C18H32CaN2O10)CONDITIONS
column
Ascentis Express RP-Amide, 5 cm x 3.0 mm I.D., 2.7 μm particles (53916-U)
mobile phase
[A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile
gradient
0% B for 0.5 min, to 15% B over 0.3 min; up to 35% B in 1.2 min, 1 min equilibration at 0% B
flow rate
2.0 mL/min
pressure
5076 psi (350 bar)
column temp.
35 °C
detector
UV, 210 nm
injection
5 μL
sample
9 vitamins in water, 10 μg/mL for nicotinic acid and folic acid, 12 μg/mL for thiamine hydrochloride and cyanocobalamin, (20 μg/mL for pyridoxine, 30 μg/mL for riboflavin, 40 μg/mL for niacineamide, 100 μg/mL for calcium pantothenate, 120 μg/mL for biotin)
Descripción
Analysis Note
A mix of 9 water-soluble B-vitamins was separated on Ascentis Express RPA column
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany