HPLC Analysis of Metoprolol Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] methanol; [B] acetic acid; [C] triethylamine; (99.8:0.1:0.1, A:B:C)

flow rate

0.5 mL/min

column temp.

20 °C

detector

UV, 225 nm

injection

2 μL

sample

1 mg/mL in methanol