HPLC Analysis of Furocoumarins on Ascentis^® Express RP-Amide

column

Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)

mobile phase

[A] water; [B] methanol; (40:60, A:B)

flow rate

1.0 mL/min

pressure

2843 psi (196 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

5 μL

sample

25 μg/mL in water:methanol (90:10)