HPLC Analysis of Benzoate and Sorbate on Ascentis® Express RP-Amide
CONDITIONS
column
Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)
mobile phase
[A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)
flow rate
0.4 mL/min
column temp.
35 °C
detector
UV, 215 nm
injection
1 μL
sample
50 mg/L each
Descripción
Analysis Note
This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of sorbate and benzoate.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany