HPLC Analysis of AQC-Amphetamine Enantiomers on Astec^® CYCLOBOND I 2000 DMP

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] 10 mM ammonium acetate, pH 4.1; [B] acetonitrile; (80:20, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

5 μL

sample

1 mg/mL in methanol