HPLC Analysis of a Peptide Drug on Antibodix^® WCX-NP10, Gradient Optimization to Separate Impurity

column

Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)

mobile phase

[A] 3 mM potassium phosphate, pH 5.0; [B] 20 mM potassium phosphate, pH 7.5

gradient

(A) 0% - 50% B (20 min); (B) 0% - 20% B (20 min), 20% B after 20 min

flow rate

1 mL/min

column temp.

ambient

detector

injection

5 μL

sample

2 mg/mL, proprietary peptide drug, pI 7-8