GC Analysis of Resveratrol in Red Wine on SLB^®-5ms after SPME using 85 μm Polyacrylate Fiber

sample/matrix

3 mL of red wine (California merlot) diluted 3:1 in 12% ethanol:water

SPME fiber

85 μm polyacrylate FS 23 gauge auto (57294-U)

SPME fiber

85 μm polyacrylate FS 24gauge auto (57305)

SPME fiber

85 μm polyacrylate FS 24gauge manual (57304)

extraction

immersion for 15 min at room temperature while stirring at 400 rpm, fiber gently blotted with a Kimwipe to remove excess water, (then derivatization by immersion in a 4 mL vial containing 5 μL of Sylon-BFT (fresh vial for each analysis than was allowed to equilabrate 60-90 minutes prior to use) for 20 min)

desorption process

280 °C for 2 min

column

SLB-5ms; 30 m x 0.25 mm I.D., 0.25 μm (28471-U)

oven

100 °C (1 min), 10 °C/min to 325 °C (3 min)

MSD interface

325 °C

scan range

m/z 40-450

carrier gas

helium, 1 mL/min, constant

liner

0.75 mm I.D., SPME