- IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design.
IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design.
The Biochemical journal (2017-08-09)
Chloe L Stoyle, Paul E Stephens, David P Humphreys, Sam Heywood, Katharine Cain, Neil J Bulleid
PMID28784690
RESUMEN
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence. Here, we show that some humanised heavy chains (HCs) can fold, form dimers and be secreted even in the absence of a light chain (LC). Quality control of recombinant antibody assembly
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Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse, clone KP-53, ascites fluid