Saltar al contenido
Merck

Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre.

Nature structural & molecular biology (2014-01-28)
Ezgi Hacisuleyman, Loyal A Goff, Cole Trapnell, Adam Williams, Jorge Henao-Mejia, Lei Sun, Patrick McClanahan, David G Hendrickson, Martin Sauvageau, David R Kelley, Michael Morse, Jesse Engreitz, Eric S Lander, Mitch Guttman, Harvey F Lodish, Richard Flavell, Arjun Raj, John L Rinn
RESUMEN

RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear-matrix factor hnRNPU through a 156-bp repeating sequence and localizes across an ~5-Mb domain on the X chromosome. We further observed Firre localization across five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X chromosome. Both genetic deletion of the Firre locus and knockdown of hnRNPU resulted in loss of colocalization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Dextran sulfate sodium salt from Leuconostoc spp., for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad
Roche
SP6 RNA Polymerase, from Escherichia coli BL 21/pSR3
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad