- Quantitative determination of urea concentrations in cell culture medium.
Quantitative determination of urea concentrations in cell culture medium.
Biochemistry and cell biology = Biochimie et biologie cellulaire (2009-05-19)
Robert J X Zawada, Peggy Kwan, Kellen L Olszewski, Manuel Llinas, Shu-Gui Huang
PMID19448747
RESUMEN
Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.
MATERIALES