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Merck

The scanning force microscopy of DNA in air and in n-propanol using new spreading agents.

FEBS letters (1994-11-21)
A Schaper, J P Starink, T M Jovin
RESUMEN

We present recent advances in DNA specimen preparation technique for scanning force microscopy (SFM) based on spreading on mica in the presence of cationic and non-ionic detergents. Reproducible DNA imaging in air and in n-propanol has been achieved in the presence of the non-ionic detergent 2,4,6-tris(dimethylaminomethyl) phenol (DMP-30) or the cationic detergent cetylpyridinium chloride (CP) in a microdrop containing nanograms of DNA. In an alternative procedure, a microdrop of detergent is applied to the surface just prior to the DNA. Quantitative image analysis yields as the apparent molecular dimensions of the DNA a width of approximately 7 nm and a height of approximately 0.7 nm, and delineates the problems of DNA metrology by SFM.

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Sigma-Aldrich
2,4,6-Tris(dimethylaminomethyl)phenol, 95%
Sigma-Aldrich
Epoxy embedding medium, accelerator, ≥95% (NT)