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  • Detection of the epidermal growth factor receptor, amphiregulin and epiregulin in formalin-fixed paraffin-embedded human placenta tissue by matrix-assisted laser desorption/ionization mass spectrometry imaging.

Detection of the epidermal growth factor receptor, amphiregulin and epiregulin in formalin-fixed paraffin-embedded human placenta tissue by matrix-assisted laser desorption/ionization mass spectrometry imaging.

European journal of mass spectrometry (Chichester, England) (2013-07-12)
Khaled Mahmoud, Laura M Cole, Jillian Newton, Sabah Mohamed, Maher Al-Enazi, Phil Quirke, Malcolm R Clench
RESUMEN

The study of the expression and the tissue distribution of the tyrosine kinase drug-target epidermal growth factor receptor (EGFR) is of interest in oncology as a marker of potential efficacy of treatment. It has been reported, however, that the response rates to anti-EGFR drugs are poorly linked to its expression. Clinical studies have also revealed a patient response correlation with the expression levels of two EGFR ligands; amphiregulin and epiregulin. Here, we report the development of a matrix-assisted laser desorption/ionisation mass spectrometry imaging methodology for the study of EGFR, epiregulin and amphiregulin distribution in formalin fixed paraffin embedded human placental tissue and a comparison to expression patterns obtained by immunohiostochemistry. Using on-tissue digests and imaging of specific peptides, the tissue distribution of these proteins has been obtained down to 30 microm spatial resolution.

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Sigma-Aldrich
Formaldehído solution, for molecular biology, 36.5-38% in H2O
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Formaldehído solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
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Epidermal Growth Factor from murine submaxillary gland, EGF, suitable for cell culture
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Formaldehído solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
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Formaldehído solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Formaldehído solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
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Formaldehído solution, 3.7% formaldehyde in H2O
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Formaldehído solution, meets analytical specification of USP, ≥34.5 wt. %
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Epidermal Growth Factor from murine submaxillary gland, lyophilized powder, BioReagent, suitable for cell culture
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Formaldehído solution, tested according to Ph. Eur.
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Formaldehído solution, SAJ first grade, ≥35.0%, contains methanol as stabilizer
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Formaldehído solution, 10%
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Formaldehído solution, JIS special grade, 36.0-38.0%, contains methanol as stabilizer
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Formaldehído solution, 7.5% formaldehyde in H2O