- Imaging of lysosomal pH changes with a fluorescent sensor containing a novel lysosome-locating group.
Imaging of lysosomal pH changes with a fluorescent sensor containing a novel lysosome-locating group.
Chemical communications (Cambridge, England) (2012-11-02)
Hao Zhu, Jiangli Fan, Qunli Xu, Honglin Li, Jingyun Wang, Pan Gao, Xiaojun Peng
PMID23114612
RESUMEN
Rlyso, a highly selective and sensitive pH sensor, can stain lysosomes with a novel lysosome-locating group, methylcarbitol. Rlyso was successfully used to detect lysosomal pH changes during apoptosis or induced by chloroquine while avoiding the "alkalizing effect" on lysosomes of current lysosomal probes with nitrogen-containing sidechains.