Saltar al contenido
Merck
  • Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4.

Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4.

Clinical proteomics (2011-09-22)
Yoshihiro Akimoto, Yuri Miura, Tosifusa Toda, Margreet A Wolfert, Lance Wells, Geert-Jan Boons, Gerald W Hart, Tamao Endo, Hayato Kawakami
RESUMEN

The objective of the present study is to identify proteins that change in the extent of the modification with O-linked N-acetylglucosamine (O-GlcNAcylation) in the kidney from diabetic model Goto-Kakizaki (GK) rats, and to discuss the relation between O-GlcNAcylation and the pathological condition in diabetes. O-GlcNAcylated proteins were identified by two-dimensional gel electrophoresis, immunoblotting and peptide mass fingerprinting. The level of O-GlcNAcylation of these proteins was examined by immunoprecipitation, immunoblotting and in situ Proximity Ligation Assay (PLA). O-GlcNAcylated proteins that changed significantly in the degree of O-GlcNAcylation were identified as cytoskeletal proteins (α-actin, α-tubulin, α-actinin 4, myosin) and mitochondrial proteins (ATP synthase β, pyruvate carboxylase). The extent of O-GlcNAcylation of the above proteins increased in the diabetic kidney. Immunofluorescence and in situ PLA studies revealed that the levels of O-GlcNAcylation of actin, α-actinin 4 and myosin were significantly increased in the glomerulus and the proximal tubule of the diabetic kidney. Immunoelectron microscopy revealed that immunolabeling of α-actinin 4 is disturbed and increased in the foot process of podocytes of glomerulus and in the microvilli of proximal tubules. These results suggest that changes in the O-GlcNAcylation of cytoskeletal proteins are closely associated with the morphological changes in the podocyte foot processes in the glomerulus and in microvilli of proximal tubules in the diabetic kidney. This is the first report to show that α-actinin 4 is O-GlcNAcylated. α-Actinin 4 will be a good marker protein to examine the relation between O-GlcNAcylation and diabetic nephropathy.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Reactivos rojos para detección in situ Duolink®
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Sonda para PLA® in situ Duolink® anti-conejo PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Sonda para PLA® in situ Duolink® anti-ratón MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Detection Reagents Orange
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Detection Reagents FarRed
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Sonda para PLA® in situ Duolink® anti-conejo MINUS
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ PLA® Probe Anti-Mouse PLUS
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Detection Reagents Green
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Wash Buffers, Fluorescence
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Mounting Medium with DAPI
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Detection Reagents Brightfield
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Probemaker PLUS
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Probemaker MINUS
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ PLA® Probe Anti-Goat PLUS
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Wash Buffer, Brightfield
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Microplate Nuclear Stain, Anti-Fade
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Duolink® In Situ Microplate Heat Transfer Block
En este momento no podemos mostrarle ni los precios ni la disponibilidad