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Merck

A Simple and Efficient CRISPR Technique for Protein Tagging.

Cells (2020-12-10)
Fanning Zeng, Valerie Beck, Sven Schuierer, Isabelle Garnier, Carole Manneville, Claudia Agarinis, Lapo Morelli, Lisa Quinn, Judith Knehr, Guglielmo Roma, Frederic Bassilana, Mark Nash
RESUMEN

Genetic knock-in using homology-directed repair is an inefficient process, requiring the selection of few modified cells and hindering its application to primary cells. Here, we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in up to 66% of transfected cells with one single electroporation. The technique has proven effective in various cell types and can be used to knock in a fluorescent protein for live cell imaging, to modify the cellular location of a target protein and to monitor the levels of a protein of interest by a luciferase assay in primary cells.

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Sigma-Aldrich
Proteína interferón-β recombinante humana, Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).