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  • Identification of cellular proteins associated with human cytomegalovirus (HCMV) DNA replication suggests novel cellular and viral interactions.

Identification of cellular proteins associated with human cytomegalovirus (HCMV) DNA replication suggests novel cellular and viral interactions.

Virology (2021-12-04)
Salomé Manska, Cyprian C Rossetto
ABSTRACT

Upon entry of Human cytomegalovirus (HCMV) into the host cell, the viral genome is transported to the nucleus where it serves as a template for transcription and genome replication. Production of new viral genomes is a coordinated effort between viral and cellular proteins. While the core replication proteins are encoded by the virus, additional cellular proteins support the process of genome synthesis. We used accelerated native isolation of proteins on nascent DNA (aniPOND) to study protein dynamics on nascent viral DNA during HCMV infection. Using this method, we identified specific viral and cellular proteins that are associated with nascent viral DNA. These included transcription factors, transcriptional regulators, DNA damage and repair factors, and chromatin remodeling complexes. The association of these identified proteins with viral DNA was confirmed by immunofluorescent imaging, chromatin-immunoprecipitation analyses, and shRNA knockdown experiments. These data provide evidence for the requirement of cellular factors involved in HCMV replication.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-RbAp48/RbAp46 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Pricing and availability is not currently available.
Sigma-Aldrich
Anti-Cytomegalovirus Antibody, early, clone 5A8.2, clone 5A8.2, Chemicon®, from mouse
Pricing and availability is not currently available.