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GE28-9924-74

HiScreen Capto Blue

Cytiva 28-9924-74, pack of 1 column

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

packaging

pack of 1 column

manufacturer/tradename

Cytiva 28-9924-74

parameter

1.2 ml/min - 2.3 ml/min flow rate
44 psi

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

capacity

~25 mg binding capacity(human serum albumin/ml medium)

storage temp.

2-8°C

General description

HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

Application

HiScreen Capto Blue is prepacked with the base matrix Capto to which Cibacron Blue 3G is covalently bound via a hydrophilic spacer immobilized with a stable amine bond. Capto Blue is highly chemically stable and has a more rigid Agarose base matrix than Blue Sepharose 6 Fast Flow. This allows the use of faster flow rates and larger sample volumes, leading to higher throughput and improved process economy. The application area is the same as for Blue Sepharose Fast FLow (e.g., purification of albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons, and related Proteins). The main benefit of Capto Blue compared to Blue Sepharose 6 Fast FLow is its improved chemical stability provided with a new coupling chemistry involving a spacer arm. It tolerates repeated cleaning-in-place and sanitization at both high and Low pH as well as autoclaving. Also the leakage of Cibacron Blue is reduced as compared to Blue Sepharose 6 Fast Flow.

Features and Benefits

  • Prepacked with Capto Blue
  • Excellent for method optimization and parameter screening due to the 10 cm bed height
  • Easily connected in series to achieve 20 cm bed height
  • Small bed volume for fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocols

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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