UPJSTAQ

Ultra pure JumpStart^™ Taq DNA Polymerase

DNA-free, Nuclease-free Hot-start Taq Polymerase, designed for further manufacture

Properties

• recombinant: expressed in E. coli expressed in
• Quality Level: 300
• form: liquid
• feature: DNA free; RNase and DNase free (also Nickase free)
• packaging: pkg of 1.5 kU
• technique(s): DNA sequencing: suitable; PCR: suitable; cDNA synthesis: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

One Unit incorporates 10nmol of total deoxyribonucleoside triphosphates into acid precipitate DNA in 30 minutes at 74°C.

Ultra pure JumpStart^™ Taq DNA Polymerase is manufactured under stringent conditions to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. The Taq DNA polymerase activity is inactivated by a neutralizing JumpStart^™ Taq antibody until the complex is dissociated at ≥70°C, providing simple and efficient hot start PCR. Hot start PCR significantly improves the results of DNA amplification by reducing the generation of nonspecific amplification products and primer-dimer artifacts. Ultra pure JumpStart^™ Taq DNA Polymerase was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet these requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.

Application

Ultra pure JumpStart^™ Taq DNA Polymerase can be used for:

• Quantitative PCR
• Multiplex PCR
• Amplification of complex genomic DNA and cDNA templates
• Amplification of low-copy number targets
• Microbiome analysis
• For further manufacture

Features and Benefits

• DNA-free
• DNase-free, RNase-free, and nickase-free
• Ideal for high throughput quantitative PCR applications
• Reduces nonspecific amplification and primer-dimers
• Increases product yield
• Allows for room-temperature PCR set-up
• Suitable for a large number of thermal cycles (>35)
• The average amplification length is 0.1 to 3kb
• Enhanced change control and quality agreement.

Packaging

Ultra pure JumpStart^™ Taq DNA Polymerase is supplied at 2.5 units/µL. By unit definition, one unit of enzyme incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74°C. Ultra pure JumpStart^™ Taq DNA Polymerase should be used with other ultra pure DNA-free reagents, such as Ultra pure 10x Taq Buffer with MgCl2, Ultra pure 10x Taq Buffer without MgCl2, Ultra pure 25 mM MgCl2, and Ultra pure DNA-free Water, to minimize false positive and negative results.

Other Notes

Quality testing ensures the absence of detectable levels of contaminating DNA (≤ 1 copy of prokaryotic gDNA, ≤ 0.1 copy of eukaryotic gDNA, and ≤ 10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable