216313
Methylene Violet (Bernthsen)
certified by the Biological Stain Commission, Dye content ≥65 %
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About This Item
Empirical Formula (Hill Notation):
C14H12N2OS
CAS Number:
Molecular Weight:
256.32
Colour Index Number:
52041
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
grade
certified by the Biological Stain Commission
Quality Level
form
powder
composition
Dye content, ≥65%
mp
216 °C (dec.) (lit.)
solubility
soluble, clear
λmax
580 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CN(C)c1ccc2N=C3C=CC(=O)C=C3Sc2c1
InChI
1S/C14H12N2OS/c1-16(2)9-3-5-11-13(7-9)18-14-8-10(17)4-6-12(14)15-11/h3-8H,1-2H3
InChI key
ALJHHTHBYJROOG-UHFFFAOYSA-N
Suitability
Certified for use as a constituent of MacNeal′s tetrachrome stain for blood.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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H Morrison et al.
Photochemistry and photobiology, 66(2), 245-252 (1997-08-01)
The interaction of methylene violet (MV) and 4-bromomethylene violet (BrMV) with calf thymus and supercoiled phi X174 phage RF I DNA is reported. Measurements employing UV-visible absorption spectroscopy and equilibrium dialysis give evidence for the formation of complexes by each
C S Sastry et al.
The Analyst, 114(4), 513-515 (1989-04-01)
A fairly sensitive spectrophotometric method for the determination of ibuprofen, ketoprofen, piroxicam, diclofenac sodium, mefenamic acid or enfenamic acid in bulk samples and pharmaceutical preparations is described, based on the formation of a chloroform-soluble, coloured ion-association complex between the drug
Jürgen Burhenne et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 273-282 (2008-02-09)
Selective and sensitive methods for the determination of the cationic dye and anti-malarial methylene blue in human liquid whole blood, dried whole blood (paper spot), and plasma depending on protein precipitation and cation exchange chromatography coupled to electrospray ionisation (ESI)
Kevin E Washburn et al.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 68(2), 105-111 (2004-06-11)
The objectives of this study were to determine the optimal concentration of phenothiazine dye required to inactivate bovine viral diarrhea virus (BVDV) in goat colostrum following 60 min of illumination and determine if immunoglobulin concentration is affected by this technique.
S J Wagner et al.
Photochemistry and photobiology, 67(3), 343-349 (1998-04-02)
A series of four phenothiazine dyes, including methylene blue (MB), were previously tested for their ability to photoinactivate viruses in red cell suspensions. One of the dyes, 1,9-dimethyl-3-dimethylamino-7-dimethylaminophenothiazine (1,9-dimethylmethylene blue), exhibited good intracellular and extracellular virucidal activity for several RNA
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