ST1200
Anti-Ubiquitin Mouse mAb (FK2)
liquid, clone FK2, Calbiochem®
Synonym(s):
Mouse Anti-Ubiquitin, Ubiquitin Detection Antibody
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
unpurified
antibody product type
primary antibodies
clone
FK2, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Immunogen
human
unpurified polyubiquitinylated lysozyme
Application
ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS.
Preparation Note
Raji cells
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Other Notes
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Amijai Saragovi et al.
eLife, 9 (2020-11-24)
Systemic oxygen restriction (SOR) is prevalent in numerous clinical conditions, including chronic obstructive pulmonary disease (COPD), and is associated with increased susceptibility to viral infections. However, the influence of SOR on T cell immunity remains uncharacterized. Here we show the
Yin Wu et al.
The Journal of biological chemistry, 291(23), 12370-12382 (2016-04-30)
Sepsis is one of the leading causes of death worldwide. Although the prevailing theory for the sepsis syndrome is a condition of uncontrolled inflammation in response to infection, sepsis is increasingly being recognized as an immunosuppressive state known as endotoxin
Juan Wang et al.
The Plant cell, 32(2), 414-428 (2019-12-13)
UBL-UBA (ubiquitin-like-ubiquitin-associated) proteins are ubiquitin receptors and transporters in the ubiquitin-proteasome system that play key roles in plant growth and development. High salinity restricts plant growth by disrupting cellular metabolism, but whether UBL-UBA proteins are involved in this process is
Tapas Mukherjee et al.
The Journal of biological chemistry, 296, 100050-100050 (2020-11-11)
Large cytosolic protein aggregates are removed by two main cellular processes, autophagy and the ubiquitin-proteasome system, and defective clearance of these protein aggregates results in proteotoxicity and cell death. Recently, we found that the eIF2α kinase heme-regulated inhibitory (HRI) induced
E Hedström et al.
Cell death & disease, 6, e1892-e1892 (2015-10-02)
Alterations in the scaffold protein WRAP53β have previously been linked to carcinogenesis and, in particular, associated with an increased risk for epithelial ovarian cancer. Here, we investigated the pathogenic impact and prognostic significance of WRAP53β in connection with epithelial ovarian
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service