RBN1MAG-31K

MILLIPLEX^® Rat Bone Magnetic Bead Panel 1 - Bone Metabolism Multiplex Assay

This MILLIPLEX MAP Rat Bone Magnetic Bead Panel 1 is a Bone Metabolism multiplex assay. The analytes available for this multiplex kit are: ACTH, Insulin, Leptin, Osteoprotegerin, DKK-1, SOST, FGF-23, PTH.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: rat
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 88-105%; sensitivity: 0.8-42.2 pg/mL; standard curve range: 0.4-1,500 pg/mL; (PTH); standard curve range: 10-40,000 pg/mL; (ACTH and OPG); standard curve range: 12-50,000 pg/mL; (DKK-1); standard curve range: 22-90,000 pg/mL; (FGF-23); standard curve range: 31-125,000 pg/mL; (Insulin); standard curve range: 5-20,000 pg/mL; (SOST); standard curve range: 7-30,000 pg/mL; (Leptin)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance, these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation.

MILLIPLEX^® Rat Bone Panel 1 is an 8-plex kit to be used for the simultaneous quantification of any or all of the following in rat serum and plasma samples: ACTH, OPG, Insulin, Leptin, PTH, DKK1, SOST, and FGF23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Specificity

Cross Reactivty
The antibody pairs in each panel do not show cross reactivity to the other analytes within the panel

Application

• Analytes: ACTH, DKK1, FGF-23, Insulin, Leptin, Osteoprotegerin (OPG), PTH, Sclerostin (SOST)
• Recommended Sample Type: Rat serum or plasma
• Recommended Sample Dilution: 25 μL per well of neat serum or plasma
• Assay Run Time: Overnight (16-18 hours) at 2-8°C is recommended; alternately incubate for 2 hours at room temperature (20-25°C).
• Research Category: Bone
• Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H315,H317,H319,H335,H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects