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Key Documents

07-1483

Sigma-Aldrich

Anti-IκBα Antibody

serum, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse, human

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NFKBIA(4792)

General description

IKK beta (IκB Kinase, IKKβ, I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. IκB Kinase (IKK) complex is a critical component in NFκB regulation. In a majority of unstimulated cells, the NFκB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IκB proteins. For NFκB to become activated, it must first disassociate from the inhibitor IκB. This occurs via the serine phosphorylation of IκB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFα, NIK, a serine/threonine kinase, activates and phosphorylates IKKα and IKKβ. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IκBα on the two serine residues. This phosphorylation marks IκB for ubiquination and destruction by the proteosome. This frees the NFκB complex and allows it to translocate to the nucleus.

Specificity

Recognizes IκBα, C-terminus. May react non-specifically with other proteins. Control peptide provided will compete only with the specific reaction of antiserum with IκBα.

Immunogen

Epitope: C-terminus
IκBα peptide corresponding to a region near the C-terminus of the human protein, conjugated to KLH.

Application

Detect IκBα using this Anti-IκBα Antibody validated for use in WB, IH(P), ELISA & IP.
Immunohistochemisty (paraffin): Representative testing from a previous lot.
Anti-IкBα staining on invasive ductal carcinoma tissue (Breast Cancer) was pretreated with citrate buffer, pH 6.0. A 1:1,000 diluted was used using IHC-Select detection with HRP-DAB.


ELISA: Recommended

Immunoprecipitation: Recommended

Optimal dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 to 1:2,000 dilution of this lot detected IkBalpha on 10 μg of HEK293 lysates.

Target description

~36 kDa

Linkage

Replaces: AB3016

Physical form

Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium phosphate with 0.15 M NaCl, pH 7.2 and 0.1% sodium azide.

Storage and Stability

Both antibody and control are stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HEK293 cell lysate.
Control Peptide: Included with the antibody is 50 μg (1 mg/mL) of IκBα control peptide. The peptide will block the specific interaction of AB3016 with the IκBα subunit. Control peptide should be used at 1.0 μg per 1.0 μL of antiserum used in assay. Optimal concentrations must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yu-Chen Hou et al.
American journal of physiology. Lung cellular and molecular physiology, 302(1), L174-L183 (2011-10-18)
Lung epithelial cells are important barriers in the respiratory system that provoke inflammatory responses through nuclear factor (NF)-κB activation to prevent pathogens from invading the body. Lipopolysaccharide (LPS) is a common pathogen-associated stimulus that activates IκB kinase (IKK) to regulate
Junyu Lai et al.
Acta cirurgica brasileira, 38, e385123-e385123 (2023-12-06)
Interstitial cystitis/bladder pain syndrome (IC/BPS) is a devastating urological chronic pelvic pain condition. In search of a potential treatment, we investigated the effect of emodin on IC/BPS inflammation and fibrosis, and explore the potential mechanism. An experimental model of interstitial
Ting Wu et al.
International journal of clinical and experimental pathology, 13(3), 437-446 (2020-04-10)
To investigate the role of an autophagy/lysosome pathway in NF-κB pathway blocked pancreatic cancer Panc-1 cells. The inhibitory effects of SN50 on pancreatic cancer cell line Panc-1 were detected by MTT assay. After SN50 treatment, autophagy activation was observed by
Qiyu Feng et al.
Nature communications, 8, 14450-14450 (2017-02-17)
Non-classical secretory vesicles, collectively referred to as extracellular vesicles (EVs), have been implicated in different aspects of cancer cell survival and metastasis. Here, we describe how a specific class of EVs, called microvesicles (MVs), activates VEGF receptors and tumour angiogenesis
María José Rodríguez et al.
Cells, 10(12) (2021-12-25)
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