Skip to Content
Merck
All Photos(6)

Documents

06-284

Sigma-Aldrich

Anti-Nitrotyrosine Antibody

Upstate®, from rabbit

Synonym(s):

Anti-Nitrotyrosine, Detection Antibody, Nitrotyrosine Detection Antibody

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

General description

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.

Specificity

Recognizes proteins which are nitrated.

Immunogen

Nitrated KLH

Application

  • Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected in fixed and permeabilized A-431 cells treated with 5mM peroxynitrite for 15 minutes.
  • Immunohistochemistry Applications: A representative lot detected Nitrotyrosine in human colon cancer, human Alzheimer′s brain and human diabetic kidney tissue sections.
  • Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
This rabbit polyclonal Anti-Nitrotyrosine, Cat. No. 06-284 is tested for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, for the detection of Nitrotyrosine.

Quality

routinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)

Target description

Predicted MW for nitrated-BSA: 85 kDa

Physical form

Format: Purified
ImmunoAffinity Purified
Purified Rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, 0.05% sodium azide, and 10 mg/mL BSA

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

D C Hooper et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(5), 691-698 (2000-04-01)
Peroxynitrite (ONOO(-)), a toxic product of the free radicals nitric oxide and superoxide, has been implicated in the pathogenesis of CNS inflammatory diseases, including multiple sclerosis and its animal correlate experimental autoimmune encephalomyelitis (EAE). In this study we have assessed
K Mikawa et al.
Anesthesiology, 94(4), 652-660 (2001-05-31)
Sepsis or peritonitis impairs diaphragmatic contractility and endurance capacity. Peroxynitrite, a powerful oxidant formed by superoxide and nitric oxide, has been implicated in the pathogenesis. Propofol scavenges this reactive molecule. The authors conducted the current study to evaluate whether propofol
S G Wilt et al.
Journal of neuroscience research, 62(3), 440-450 (2000-10-31)
Vacuolation in cellular organelles within the central nervous system is a common manifestation of oxidative injury. We found that the spongiform vacuolation observed in PVC-211 murine leukemia virus (PVC-MuLV) neurodegeneration was associated with oxidative damage as detected by immunoreactivity for
Nitric oxide affects the phosphorylation state of microtubule-associated protein 2 (MAP-2) and neurofilament: an immunocytochemical study in the brain of rats and neuronal nitric oxide synthase (nNOS)-knockouts.
Fritz Rothe, Heiko Possel, Gerald Wolf
Nitric Oxide null
S K Wattanapitayakul et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(2), 271-278 (2000-02-05)
Angiotensin II (ANG II) is a well-established participant in many cardiovascular disorders, but the mechanisms involved are not clear. Vascular cell experiments suggest that ANG II is a potent stimulator of free radicals such as superoxide anion, an agent known

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service