05-224
Anti-p53 Antibody, clone BP53-12
clone BP53-12, Upstate®, from mouse
Synonym(s):
Anti-BCC7, Anti-BMFS5, Anti-LFS1, Anti-P53, Anti-TRP53
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BP53-12, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μL
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.
Specificity
Recognizes p53.
Immunogen
Recombinant human wild type p53
Application
Anti-p53 Antibody, clone BP53-12 is a high quality Mouse Monoclonal Antibody for the detection of p53 & has been validated in IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
routinely evaluated on RIPA lysate of human Raji cells
Target description
53 kDa
Linkage
Replaces: 04-1083
Physical form
Format: Purified
Protein A purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Raji cell lysate
Raji cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The estrogen receptor alpha pathway induces oncogenic Wip1 phosphatase gene expression.
Hye-Sook Han, Eunsil Yu, Ji-Young Song, Ji-Young Park, Se Jin Jang, Jene Choi et al.
Molecular Cancer Research null
Translocation of p53 to mitochondria is regulated by its lipid binding property to anionic phospholipids and it participates in cell death control.
Ching-Hao Li,Yu-Wen Cheng,Po-Ling Liao,Jaw-Jou Kang
Neoplasia null
Jooyeon Kim et al.
Stem cells and development, 24(5), 575-586 (2014-12-02)
Human umbilical cord mesenchymal stromal cells (hUC-MSCs) of Wharton's jelly origin undergo adipogenic, osteogenic, and chondrogenic differentiation in vitro. Recent studies have consistently shown their therapeutic potential in various human disease models. However, the biological effects of major pregnancy complications
F Wang et al.
Oncogene, 35(47), 6087-6095 (2016-05-03)
SALL4 is aberrantly expressed in human myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). We have generated a SALL4 transgenic (SALL4B Tg) mouse model with pre-leukemic MDS-like symptoms that transform to AML over time. This makes our mouse model applicable
J Liu et al.
The Journal of biological chemistry, 275(16), 11846-11851 (2000-04-15)
Stimulation of transfected HepG2 cells (TFG2) with the alpha(1)-adrenergic agonist phenylephrine (PE) significantly activated p21(waf1/cip1) gene expression without affecting p53 gene expression. Northern blotting and reporter assay demonstrated that this induction was due to PE stimulation of p21(waf1/cip1) mRNA stability.
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