Skip to Content
Merck
All Photos(3)

Documents

05-1341

Sigma-Aldrich

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301

clone CMA301, from mouse, purified by affinity chromatography

Synonym(s):

H3 histone family, member T, histone 3, H3, histone cluster 3, H3

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity purified immunoglobulin

clone

CMA301, monoclonal

purified by

affinity chromatography

species reactivity

vertebrates, human

technique(s)

ELISA: suitable
dot blot: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Specificity

Broad species cross-reactivity is expected, based on sequence homology.
This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.

Immunogen

Epitope: N-terminus (unmodified)
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.

Application

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.

Quality

Routinely evaluated by Western Blot on HeLa cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 μg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.

Target description

Approx. 17 kDa

Physical form

Mouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.
Peptide affinity chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa Acid extract lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Martin L Schmitt et al.
Journal of biomolecular screening, 19(6), 973-978 (2014-04-02)
Posttranslational modifications of histone tails are very important for epigenetic gene regulation. The lysine-specific demethylase LSD1 (KDM1A/AOF2) demethylates in vitro predominantly mono- and dimethylated lysine 4 on histone 3 (H3K4) and is a promising target for drug discovery. We report
Xiaojuan Liu et al.
The Journal of biological chemistry, 293(38), 14669-14677 (2018-08-08)
Hypoxia-inducible factor 1α (HIF-1α) is a transcription factor that regulates cellular responses to hypoxia. It controls the expression of both BCL2/adenovirus E1B 19-kDa protein-interacting protein 3 (BNIP3) and insulin-like growth factor 2 (IGF2). Previous studies have demonstrated that in hypoxia
Impaired Experience-Dependent Refinement of Place Cells in a Rat Model of Alzheimer's Disease.
Broussard, et al.
Journal of Alzheimer'S Disease, 86, 1907-1916 (2023)
ADAM17-mediated CD44 cleavage promotes orasphere formation or stemness and tumorigenesis in HNSCC.
Kamarajan, P; Shin, JM; Qian, X; Matte, B; Zhu, JY; Kapila, YL
Cancer Medicine null
Genome-wide analysis of the chromatin sites targeted by HEX 70a storage protein in the honeybee brain and fat body.
Martins, et al.
Insect Molecular Biology, 32, 277-304 (2023)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service