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Supelco

SUPELCOSIL LC-8 (3 µm) HPLC Columns

L × I.D. 7.5 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

Columna para HPLC SUPELCOSIL LC-8, 3 μm particle size, L × I.D. 7.5 cm × 4.6 mm

agency

suitable for USP L7

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

6.0% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

7.5 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.2 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C8 (octyl) phase

particle size

3 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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F Belal et al.
Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
S B Ruddy et al.
Journal of chromatography. B, Biomedical applications, 657(1), 83-92 (1994-07-01)
The isocratic, reversed-phase, high-performance liquid chromatographic (HPLC) method presented provides a simple and rapid analytical technique for the simultaneous determination of low-molecular-mass oligomers of polyethylene glycol (PEG) in aqueous polymer samples containing polar contaminants of biologic origin. In the present
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions

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