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L5520

Sigma-Aldrich

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Leibovitz L15

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About This Item

MDL number:
MFCD00217482
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells
  • as a component of culture buffer for the tissue culture of Newt lung cells

Reconstitution

Supplement with 0.3 g/L L-glutamine.

also commonly purchased with this product

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related product

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supplement

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Francisco J Monje et al.
Annals of medicine, 45(4), 301-307 (2013-05-02)
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Olga Krylyshkina et al.
The Journal of cell biology, 156(2), 349-359 (2002-01-25)
Recent studies have shown that the targeting of substrate adhesions by microtubules promotes adhesion site disassembly (Kaverina, I., O. Krylyshkina, and J.V. Small. 1999. J. Cell Biol. 146:1033-1043). It was accordingly suggested that microtubules serve to convey a signal to
Applications of cell lines as bioreactors and in vitro models
Singh J and Goswami A
International journal of applied & basic medical research null
B Houchmandzadeh et al.
The Journal of cell biology, 139(1), 1-12 (1997-10-06)
The structure of mitotic chromosomes in cultured newt lung cells was investigated by a quantitative study of their deformability, using micropipettes. Metaphase chromosomes are highly extensible objects that return to their native shape after being stretched up to 10 times
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