391340

HEPES

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

• Sinónimos: Ácido 4-(2-hidroxietil)piperacina-1-etanosulfónico, Ácido N-(2-hidroxietil)piperacina-N′-(2-etanosulfónico)
• Fórmula empírica (notación de Hill): C₈H₁₈N₂O₄S
• Número de CAS: 7365-45-9
• Peso molecular: 238.30
• Beilstein/REAXYS Number: 883043
• MDL number: MFCD00006158
• UNSPSC Code: 12161704
• NACRES: NA.25

Propiedades

• product name: HEPES, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
• grade: Molecular Biology
• Quality Level: 100
• assay: ≥99% (alkalimetric)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 400 mg/mL; aqueous base: soluble
• cation traces: heavy metals: ≤0.0005%
• foreign activity: DNase, RNase, and protease, none detected
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descripción

General description

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

Application

• Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable