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Key Documents

1.02572

Sigma-Aldrich

Schiff′s reagent Intense

for the detection of aldehyde and mucosubstances in microscopy

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About This Item

UNSPSC Code:
41116130
NACRES:
NA.41

Quality Level

form

liquid

IVD

for in vitro diagnostic use

pH

1.74 ( in H2O)

density

1.014 g/cm3 at 20 °C

storage temp.

20-25°C

General description

The Schiff′s reagent Intense - for the detection of aldehyde and mucosubstances in microscopy, is a ready-to-use solution used for human-medical cell diagnosis and serves the histological investigation of sample material of human origin, for example histological sections of e. g. the intestine or liver. This ready-to-use Schiff′s reagent Intense can be used to stain mucopolysaccharides in histological tissue specimens. The PAS (periodic acid schiff) reaction is one of the most frequently used chemical methods for the detection of aldehyde and mucosubstances in microscopy. In the PAS reaction, the histological specimen material is first treated with periodic acid, resulting in the oxidation of the 1,2-glycols into aldehyde groups. The addition of Schiff′s reagent (fuchsin-sulfuric acid) in the second step causes the aldehydes to react to form a brilliant red color. In the end result, the PAS reaction yields a specific color reaction with unsubstituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glyco- and phospholipids.
The PAS reaction can be further combined with the Alcian blue staining method to detect mucosubstances (glycosaminoglycans).
The 500 ml bottle is sufficient for 3000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For the detection of aldehydes, polysaccharides (glycogen), and mucosubstances (glycoproteins, glycolipids, mucins)

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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