R4279

Anti-RbAp46, C-terminal antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-RBBP7, Anti-Retinoblastoma-binding protein 7
• MDL number: MFCD08705513
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 46 kDa
• species reactivity: human
• technique(s): indirect immunofluorescence: 2-4 μg/mL using human HeLa cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using nuclear extracts of human HeLa cells
• UniProt accession no.: Q16576
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RBBP7(5931)

Description

General description

Retinoblastoma-associated binding protein 46 (RbAp46) is also termed as retinoblastoma-binding protein 7 (RBBP7) and has 89% amino acid identity with RbAp48. It belongs to the WD-repeat protein family and has four repeats that ends with Trp-Asp(WD) residues. This protein is a vital component of the histone modifying and remodeling complexes. RBBP7 is mapped to human chromosome Xp22.13

Specificity

Anti-RbAp46, C-terminal specifically recognizes human RbAp46.

Immunogen

synthetic peptide corresponding to amino acids 411-425 of human RbAp46, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in human and differs from the mouse sequence by one amino acid.

Application

Anti-RbAp46, C-terminal antibody produced in rabbit has been used in western blotting and immunofluorescence.

Biochem/physiol Actions

Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in modification and remodeling of chromatin during cell growth and differentiation. It plays a key role in the nucleosomal remodeling and deacetylation complex (NURD). It may also involve in histone deacetylation and transcription repression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves