A7356

Anti-Atg16L antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

• Synonym(s): Anti-APG16L, Anti-ATG16 autophagy related 16-like 1 (S. cerevisiae), Anti-ATG16L1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa (several isonform bands)
• species reactivity: rat, mouse, human
• technique(s): western blot: 1:500-1:1,000 using whole extracts of human A549 cells, rat PC12 cells, and HEK-293T cells expressing mouse Atg16l
• UniProt accession no.: Q676U5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATG16L1(55054) ; mouse ... Atg16l1(77040) ; rat ... Atg16l1(363278)

Description

General description

ATG16L is a component of a large protein complex that is critical for autophagy.

Autophagy-related 16-like 1 (ATG16L1) is an autophagy gene, that is expressed in the colon, small intestine, intestinal epithelial cells, leukocytes and spleen. Atg16L is expressed in different isoform patterns depending on the tissue.

Immunogen

synthetic peptide correspopnding to amino acids 2-15 of mouse Atg16l, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and human.

Application

Anti-Atg16L antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Atg16L is involved in LC3 lipidation during the formation of autphagosomes. Studies have reported that Atg16L may also function as a mammalian Rab33 effector.

Autophagy-related 16-like 1 (ATG16L1) modulates host immune responses and is linked with several diseases, like cardiovascular disease (CVD). Atg16 multimeric complex plays an essential role in autophagy. Atg16L interacts with both Atg5 and additional Atg16L monomers. Together with Atg12-Atg5 conjugate, Atg16L associates with the autophagic isolation membrane for the duration of autophagosome formation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves