361551
GSK-3 Inhibitor X
The GSK-3 Inhibitor X, also referenced under CAS 740841-15-0, controls the biological activity of GSK-3. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
Synonym(s):
GSK-3 Inhibitor X, (2ʹZ,3ʹE)-6-Bromoindirubin-3ʹ-acetoxime, BIO-Acetoxime
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About This Item
Empirical Formula (Hill Notation):
C18H12BrN3O3
CAS Number:
Molecular Weight:
398.21
UNSPSC Code:
12352200
NACRES:
NA.77
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Quality Level
Assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
dark purple
solubility
DMSO: 10 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
An acetoxime analog of BIO, GSK-3 Inhibitor IX (Cat. No. 361550) that exhibits greater selectivity for GSK-3α/β (IC50 = 10 nM) over Cdk5/p25, Cdk2/A and Cdk1/B (IC50 = 2.4 µM, 4.3 µM and 63 µM, respectively). Weakly affects the activities of Cdk4/D1 and many other kinases (IC50 ≥ 10 µM). Also acts as an aryl hydrocarbon receptor agonist in both yeast (EC50 ≥ 0.1 µM) and mammalian (EC50 = 0.16 µM) reporter systems.
An acetoxime analog of BIO, GSK-3 Inhibitor IX (Cat. No. 361550) that exhibits greater selectivity for GSK-3α/β (IC50 = 10 nM) over Cdk5/p25, Cdk2/cyclin A, and Cdk1/cyclin B (IC50 = 2.4 µM, 4.3 µM, and 63 µM, respectively). A weak inhibitor of Cdk4/cyclin D1 and many other non-related kinases (IC50 ≥ 10 µM). Yields BIO (Cat. No. 361550) upon hydrolysis.
Biochem/physiol Actions
Cell permeable: no
Primary Target
GSK-3α/β
GSK-3α/β
Product does not compete with ATP.
Reversible: no
Target IC50: 10 nM against GSK-3α/β
Packaging
Packaged under inert gas
Warning
Toxicity: Carcinogenic / Teratogenic (D)
Preparation Note
Limited solubility in aqueous buffers; serial dilutions in DMSO may be required prior to dilution in buffer.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Other Notes
Knockaert, M., et al. 2004. Oncogene23, 4400.
Polychronopoulos, P., et al. 2004. J. Med. Chem.47, 935.
Meijer, L., et al. 2003. Chem. Biol.10, 1255.
Polychronopoulos, P., et al. 2004. J. Med. Chem.47, 935.
Meijer, L., et al. 2003. Chem. Biol.10, 1255.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Noboru Sato et al.
Methods in molecular biology (Clifton, N.J.), 331, 115-128 (2006-08-03)
This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating
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