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Vitamin D Binding Protein Isoforms and Apolipoprotein E in Cerebrospinal Fluid as Prognostic Biomarkers of Multiple Sclerosis.

PloS one (2015-06-06)
Simona Perga, Alessandra Giuliano Albo, Katarzyna Lis, Nicoletta Minari, Sara Falvo, Fabiana Marnetto, Marzia Caldano, Raffaella Reviglione, Paola Berchialla, Marco A Capobianco, Maria Malentacchi, Davide Corpillo, Antonio Bertolotto
RÉSUMÉ

Multiple sclerosis (MS) is a multifactorial autoimmune disease of the central nervous system with a heterogeneous and unpredictable course. To date there are no prognostic biomarkers even if they would be extremely useful for early patient intervention with personalized therapies. In this context, the analysis of inter-individual differences in cerebrospinal fluid (CSF) proteome may lead to the discovery of biological markers that are able to distinguish the various clinical forms at diagnosis. To this aim, a two dimensional electrophoresis (2-DE) study was carried out on individual CSF samples from 24 untreated women who underwent lumbar puncture (LP) for suspected MS. The patients were clinically monitored for 5 years and then classified according to the degree of disease aggressiveness and the disease-modifying therapies prescribed during follow up. The hierarchical cluster analysis of 2-DE dataset revealed three protein spots which were identified by means of mass spectrometry as Apolipoprotein E (ApoE) and two isoforms of vitamin D binding protein (DBP). These three protein spots enabled us to subdivide the patients into subgroups correlated with clinical classification (MS aggressive forms identification: 80%). In particular, we observed an opposite trend of values for the two protein spots corresponding to different DBP isoforms suggesting a role of a post-translational modification rather than the total protein content in patient categorization. These findings proved to be very interesting and innovative and may be developed as new candidate prognostic biomarkers of MS aggressiveness, if confirmed.

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Description du produit

Sigma-Aldrich
Dibutyl phthalate, 99%
Sigma-Aldrich
Phosphate de sodium dibasic dodecahydrate, BioXtra, ≥99.0% (T)
Sigma-Aldrich
Aprotinine from bovine lung, lyophilized powder, 3-7 TIU/mg solid
Sigma-Aldrich
DBP, 98% (HPLC)
Sigma-Aldrich
Anti-Vitamin D-Binding Protein/GC antibody produced in goat, affinity isolated antibody, buffered aqueous solution