Accéder au contenu
Merck

Isolation, chemical characterization, and free radical scavenging activity of phenolics from Triticum aestivum L. aerial parts.

Journal of agricultural and food chemistry (2014-10-31)
Iwona Kowalska, Lukasz Pecio, Lukasz Ciesla, Wieslaw Oleszek, Anna Stochmal
RÉSUMÉ

Fourteen phenolic compounds (flavonoids and phenolic acids) were isolated and 19 were identified in the aerial parts of Triticum aestivum L. The structures of these compounds were established on the basis of the data obtained by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) techniques. T. aestivum L. was found to be rich in flavones, especially in luteolin derivatives. Three of the isolated compounds, including luteolin 6-C-[6Glc″-O-E-caffeoyl-β-D-glucopyranosyl(1″→2)-β-glucopyranoside], luteolin 6-C-[5Rib″-O-E-feruoyl-β-D-ribofuranosyl(1″→2)-β-glucopyranoside], and 3',4',5'-O-trimethyltricetin 7-O-[β-D-glucuropyranosyl(1″→2)-β-D-glucopyranoside], have been reported for the first time in the plant kingdom. The amount of individual phenolics, in winter wheat, was also determined. Additionally, the free radical scavenging potential of the isolated compounds was tested in a simple and rapid thin-layer chromatography-2,2-diphenyl-1-picrylhydrazyl radical test (TLC-DPPH•) with image processing.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Acétonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Méthanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Méthanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Acétate d'éthyle, ACS reagent, ≥99.5%
Sigma-Aldrich
Sulfoxyde de diméthyle-d6, Diméthylsulfoxyde-d6, 99.9 atom % D
Sigma-Aldrich
Acide acétique, glacial, ACS reagent, ≥99.7%
Sigma-Aldrich
Acétonitrile, HPLC Plus, ≥99.9%
Sigma-Aldrich
Acide acétique, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
Méthanol-d4, ≥99.8 atom % D
Sigma-Aldrich
Méthanol, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Acétate d'éthyle, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Chloroforme, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
Toluène, ACS reagent, ≥99.5%
Sigma-Aldrich
Acide formique, reagent grade, ≥95%
Sigma-Aldrich
Acide phosphorique, ACS reagent, ≥85 wt. % in H2O
Sigma-Aldrich
Chloroforme, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Sigma-Aldrich
Méthanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Acétate d'éthyle, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%
Sigma-Aldrich
Toluène, suitable for HPLC, 99.9%
Sigma-Aldrich
Chloroforme, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Acide formique, ACS reagent, ≥96%
Sigma-Aldrich
Acide formique, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
Sigma-Aldrich
Toluène, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Sulfoxyde de diméthyle-d6, Diméthylsulfoxyde-d6, 99.9 atom % D, contains 0.03 % (v/v) TMS
Sigma-Aldrich
Acétonitrile, ACS reagent, ≥99.5%
Sigma-Aldrich
2,2-Diphenyl-1-picrylhydrazyl
Sigma-Aldrich
Chloroforme, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Sigma-Aldrich
Acétonitrile, for HPLC, for UV, ≥99.9% (GC)
Sigma-Aldrich
Acétonitrile, anhydrous, 99.8%
Sigma-Aldrich
Acide phosphorique, 85 wt. % in H2O, 99.99% trace metals basis