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Protective effects of selenium against sister chromatid exchange induced by AFG 1 in human lymphocytes in vitro.

Human & experimental toxicology (2010-07-16)
Lokman Alpsoy, Elif Kotan, Abdulgani Tatar, Guleray Agar
RÉSUMÉ

Aflatoxins have been shown to be hepatotoxic, carcinogenic, mutagenic and teratogenic to different species of animals. Besides, at low concentrations, Selenium (Se(4+)) is antimutagenic and anticarcinogenic while it is toxic, mutagenic and carcinogenic at high concentrations. In this study, we aimed to evaluate the effect of Se(4+) against aflatoxin GAFG(1) (AFG(1)) on blood cultures in relation to induction of sister chromatid exchange (SCE). The results showed that at 0.4 and 0.8 parts per million (ppm) concentration of AFG(1), the frequency of SCE increased in cultured human lymphocytes. When different concentration of Se(4+) (0.08 and 8 ppm) were added to AFG(1), the frequencies of SCE decreased. Howewer, when 800 ppm concentration of Se(4+) together with 0.08 ppm AFG(1) were added to cell division inhibited in the cultures. Results suggested that Se(4+) could effectively inhibit AFG(1)-induced SCE. Besides, the protective role of Se(4+) against AFG(1)-induced SCE is probably related to its doses.

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Sigma-Aldrich
Aflatoxin G1, from Aspergillus flavus
Supelco
Aflatoxine G1 solution, 2 μg/mL in acetonitrile, analytical standard
Aflatoxine G1 solution, 3.78 μg/g in acetonitrile, ERM®, certified reference material
Supelco
Aflatoxin G1