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Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays.

Bio-protocol (2021-10-26)
Mark W Soo, Arneet L Saltzman
RÉSUMÉ

In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more modifications are a key tool to probe these interactions in pull-down assays with recombinant proteins or cell lysates. Building on these approaches, the binding specificity of a protein of interest can be screened against many histone peptides in parallel using a peptide array. In this protocol, we describe the expression and purification of a recombinant protein of interest in bacteria, followed by an assay for binding to histone post-translational modifications using a commercially available histone peptide array. The purification uses a versatile dual-tagging and cleavage strategy and equipment commonly available in a molecular biology laboratory. Graphic abstract: Overview of protocol for purifying recombinant protein and hybridizing to a histone peptide array.

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Sigma-Aldrich
Albumine de sérum bovin, heat shock fraction, pH 7, ≥98%
Sigma-Aldrich
Nalgene® centrifuge tubes, Oak Ridge Style 3114, capacity 50 mL, pack of 2 ea