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Explant Methodology for Analyzing Neuroblast Migration.

Bio-protocol (2017-07-21)
Kirsty J Dixon, Alisa Turbic, Ann M Turnley, Daniel J Liebl
RÉSUMÉ

The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we describe the isolation of SVZ-derived RMS explants from the neonatal mouse brain, and the conditions required to culture and evaluate their migration.

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Sigma-Aldrich
Hydroxyde de sodium, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
Sérum d'âne
Pipette filter tips, universal fit, racked, size 1000 μL, pkg of 5x1000tips/cs, sterile