X4875

Xanthine Oxidase from bovine milk

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

• Synonyme(s) : XOD, Xanthine:oxygen oxidoreductase
• Numéro CAS: 9002-17-9
• Numéro de classification (Commission des enzymes): 1.17.3.2.
• Numéro CE : 232-657-6
• Numéro MDL: MFCD00082145
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine milk
• Type: Grade IV
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥0.1 units/mg protein
• Température de stockage: 2-8°C

Description

Description générale

Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.

enthält auch proteolytische Aktivität

Application

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Actions biochimiques/physiologiques

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Définition de l'unité

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Forme physique

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Remarque sur l'analyse

Protein determined by biuret

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)