SAB5700591

Recombinant 2019-nCoV Spike S1 Protein with hFc and His tag

• Synonyme(s) : S glycoprotein Subunit1, S1 protein, Spike glycoprotein Subunit1, Spike protein S1, novel coronavirus s1 Protein
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.26

Propriétés

• Produit recombinant: expressed in HEK 293 cells
• Niveau de qualité: 100
• Pureté: >95% (SDS-PAGE)
• Forme: lyophilized powder
• Poids mol.: 103 kDa
• Impuretés: <0.1 EU/μg protein (LAL test)
• Numéro d'accès UniProt: P0DTC2
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: SARS virus ... S(43740568)

Description

Description générale

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.

Actions biochimiques/physiologiques

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay.

Reconstitution

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Stockage et stabilité

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable