Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
9004-54-0
Numéro MDL:
MFCD00132771
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.52

Qualité

DNA grade
for molecular biology

Gamme de produits

BioReagent

Forme

beads

Technique(s)

DNA purification: suitable

Matrice

cross-linked dextran

Gonflement

1 g swells to 9-11 mL

Diamètre des billes

20-80 μm

Dimension de pores

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

Activité étrangère

DNase, RNase, none detected

Température de stockage

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

Clé InChI

FZWBNHMXJMCXLU-UHFFFAOYSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Caractéristiques et avantages

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 6

1 of 6

Sephadex® G-50 DNA grade, Cytiva, pack of 25 g

GE17-0573-01

Sephadex® G-50

Sephadex® G-50 M DNA grade

GE17-0045-02

Sephadex® G-50

Sephadex® G-50 Medium, Cytiva, pack of 500 g

GE17-0043-02

Sephadex® G-50

Sephadex® G-50 Superfine, Cytiva, pack of 100 g

GE17-0041-01

Sephadex® G-50

Sephadex® G-100 DNA Grade 100 g Cytiva 17-0574-02

GE17-0574-02

Sephadex® G-100 DNA Grade 100 g

Sephadex® G-75 Superfine

Sigma-Aldrich

G7550

Sephadex® G-75

Torben Riehl et al.
PloS one, 7(11), e49354-e49354 (2012-11-13)
The Amundsen Sea, Antarctica, is amongst the most rapidly changing environments of the world. Its benthic inhabitants are barely known and the BIOPEARL 2 project was one of the first to biologically explore this region. Collected during this expedition, Macrostylis
Aron Fazekas et al.
BioTechniques, 48(4), 277-285 (2010-06-24)
Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique