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RDD002

Sigma-Aldrich

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri™

Synonyme(s) :

Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)

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About This Item

Formule empirique (notation de Hill):
C8H18N2O4S
Numéro CAS:
7365-45-9
Poids moléculaire :
238.30
Numéro Beilstein :
883043
Numéro CE :
230-907-9
Numéro MDL:
MFCD00006158
Code UNSPSC :
12161700
ID de substance PubChem :
329824215
Nomenclature NACRES :
NA.25

product name

HEPES, anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Qualité

anhydrous
free-flowing

Niveau de qualité

100

Gamme de produits

Redi-Dri™

Pureté

≥99.5%

Forme

crystalline powder

pH

5.0-6.5 (25 °C, 238 g/L)

Plage de pH utile

6.8-8.2

pKa (25 °C)

7.5

Solubilité

water: 500 mg/mL, clear, colorless

Chaîne SMILES 

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

Clé InChI

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Description générale

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
  • As a component in intracellular solution for the preparation of brain-slice recording
  • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
  • To maintain the pH at 7 in thermal denaturation assay of purified protein
  • In isocratic elution of protein using gel filtration
  • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Informations légales

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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HEPES hemisodium salt dry powder

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HEPES A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

Millipore

391340

HEPES

HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

Solution tampon HEPES BioUltra, for molecular biology, 2× concentrate

Sigma-Aldrich

51558

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HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

H3784

HEPES sodium salt

Cell-free Generation of COPII-coated Procollagen I Carriers.
Yuan L, et al.
Bio-protocol, 7(22) (2017)
Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations.
Mingote S, et al.
The Journal of Neuroscience (2015)
Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.
Koppel N, et al.
eLife, 7, e33953-e33953 (2018)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)
Hydrogen ion buffers for biological research.
Good NE, et al.
Biochemistry, 5(2), 467-477 (1966)
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Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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