M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

• Synonyme(s) : Moloney Murine Leukemia Virus Reverse Transcriptase
• Numéro CAS: 9068-38-6
• Numéro MDL: MFCD00283754
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.55

Propriétés

• Source biologique: Porcine intestinal mucosa
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Forme: liquid
• Utilisation: sufficient for 200 reactions; sufficient for 250 reactions
• Caractéristiques: dNTPs included: no; hotstart: no
• Concentration: 200 units/μL
• Technique(s): RT-PCR: suitable
• Couleur: colorless
• Entrée: purified RNA
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues.[1] It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Application

M-MLV Reverse Transcriptase has been used:

• for the preparation of cDNA libraries or for first strand cDNA synthesis[2][3]
• for use in a 2-step RT-PCR assay[4][5][6]
• in quantitative realtime-polymerase chain reaction (RT-qPCR)[5]
• in reverse transcription[6]

Caractéristiques et avantages

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.

Conditionnement

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Définition de l'unité

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Notes préparatoires

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Informations légales

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)